The true story of Spectral flow cytometry

Some recent publications on spectral cytometry and some companies seem to ignore a part of the story about spectral flow cytometry and in particular the work done by Professor Robinson’s group at Purdue University back in the early 2000s. To help the readers to know what is the story, here is a summary of our presentations :

 Our first work on spectral flow cytometry was presented at the 2004 ISAC conference “Collection Hardware for high speed mutlispectral single particle analysis”.

 Weeks prior to that conference (April 4, 2004) we submitted a US patent application (“Multispectral detector and analysis system”) resulting in # US7,280,204 issued on Oct 9, 2007 with a priority date of April 8, 2004.

 A rapid publication was also made in Biophotoncis International , Oct, 2004 “Multispectral Cytometry : The Next Generation” (copy attached) – this was the first paper showing the current implementation of spectral flow cytometry.

 Then a note in Microsc Microanal 11(Suppl 2) was published in 2005 : “Multispectral Flow Cytometry : Next Generation tools for Automated Classification” DOI : 10.1017/S1431927605510328.

 We also published a SPIE paper : (Advanced Biomedical and Clinical Diagnostic Systems III, edited by Tuan Vo-Dinh, Warren S. Grundfest, David A. Benaron, Gerald E. Cohn, Proc. of SPIE Vol. 5692) entitled “Multispectral cytometry of single bio-particles using a 32-channel detector”.

 We also published an article in Cytometry 81A:35-44, 2012 “Hyperspectral cytometry at the single cell level using a 32-Channel Photodetector”.

Now you have all the information and know the full story of this work which was patented by Purdue University, and licensed to Sony to produce the first commercial spectral flow cytometer.